Catalogue / Tirzepatide
Tirzepatide
Also written as GIP/GLP-1 dual agonist.
A 39 residue dual GIP and GLP-1 receptor agonist carrying a C20 diacid on lysine 20, at nearly 4.8 kilodaltons the largest of the commonly submitted incretin analogues.
| Molecular formula | C225H348N48O68 |
| Average mass | 4813.45 Da |
| Monoisotopic mass | 4810.5249 Da |
| Class | Incretin analogues |
| Determination | Blind identification, GLP-1 class |
| Price | 468 USD |
Computed at build time from the composition shown on this page, not transcribed from a supplier datasheet.
Tirzepatide has an average molecular weight of 4813.45 daltons and a monoisotopic mass of 4810.5249 daltons. Identity is confirmed by matching the measured deconvoluted mass to that figure, and purity is reported separately by reversed-phase chromatography at 214 nm. The two numbers answer different questions and neither substitutes for the other.
| Charge | m/z |
|---|---|
| [M+3H]3+ | 1604.516 |
| [M+4H]4+ | 1203.638 |
| [M+5H]5+ | 963.112 |
These are the ions an electrospray source is expected to produce for a neutral mass of 4810.5249 daltons, computed from the monoisotopic mass and the proton mass. They are what a raw spectrum should show before deconvolution.
A report that gives only a deconvoluted mass without the charge states it was derived from cannot be checked. Deconvolution is a calculation, and a calculation whose inputs are not shown is an assertion.
| At this mass the isotope envelope is wide and the monoisotopic peak is no longer the most abundant. Reporting the average mass alongside the monoisotopic mass avoids a false 3 to 4 dalton discrepancy. |
| Truncated syntheses missing one or two C-terminal residues are chromatographically close and are the most common real impurity in this class. |
| The compound is frequently submitted blind. Confirming it means excluding every other candidate in the class on mass, not matching one standard. |
Every compound fails in its own way. A generic peptide method applied without regard to these specifics produces a number that looks like a result and is not one.
Long-acting GLP-1 and dual agonists carrying a fatty acid chain on a lysine side chain. The class is chemically crowded, and analogues differ by a single substitution or a linker length.
LC-MS on the multiply charged envelope with deconvolution to the neutral mass, because analogues in this class co-elute closely and a retention-time match cannot separate them. Purity by RP-HPLC at 214 nm with a shallow acetonitrile gradient.
Where the delivered milligram figure matters rather than the percentage, net peptide content by combustion analysis and water by Karl Fischer are added, and the certificate carries a closed mass balance instead of a single purity number.
| LC-MS identity | Measured mass |
| RP-HPLC purity | Area at 214 nm |
| Net peptide content | Milligrams |
| Water content | Karl Fischer |
| Counter-ion | Ion chromatography |
| Bacterial endotoxins | USP <85> |
What is the molecular weight of Tirzepatide?
The average molecular weight is 4813.45 daltons and the monoisotopic mass is 4810.5249 daltons, computed from the molecular formula C225H348N48O68 shown on this page. Both figures are calculated rather than transcribed, so they can be checked against the stated composition.
How is Tirzepatide tested for purity and identity?
LC-MS on the multiply charged envelope with deconvolution to the neutral mass, because analogues in this class co-elute closely and a retention-time match cannot separate them. Purity by RP-HPLC at 214 nm with a shallow acetonitrile gradient. Purity describes the chromatographable fraction only and is not the same quantity as the peptide mass delivered per vial.
What goes wrong when Tirzepatide is analysed?
At this mass the isotope envelope is wide and the monoisotopic peak is no longer the most abundant. Reporting the average mass alongside the monoisotopic mass avoids a false 3 to 4 dalton discrepancy.
What does Tirzepatide analysis cost at ANALYTON?
Blind identification, GLP-1 class is priced at 468 US dollars, covering the determinations described on this page. Content, safety and elemental determinations are ordered alongside and are priced separately on the price list.
Other compounds in this class
Updated 2026-09-01. Masses computed at build, not transcribed.