Catalogue / Melanotan I

Melanotan I

Also written as Afamelanotide, MT-1.

A thirteen residue linear analogue of alpha-MSH with norleucine at position 4 and D-phenylalanine at position 7, acetylated and amidated.

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Computed constants
Molecular formula C78H111N21O19
Average mass 1646.85 Da
Monoisotopic mass 1645.8365 Da
Written as Ac-Ser-Tyr-Ser-Nle-Glu-His-D-Phe-Arg-Trp-Gly-Lys-Pro-Val-NH2
ClassMelanocortin analogues
DeterminationPeptide analysis, specialist compound
Price 590 USD

Computed at build time from the composition shown on this page, not transcribed from a supplier datasheet.

Melanotan I has an average molecular weight of 1646.85 daltons and a monoisotopic mass of 1645.8365 daltons. Identity is confirmed by matching the measured deconvoluted mass to that figure, and purity is reported separately by reversed-phase chromatography at 214 nm. The two numbers answer different questions and neither substitutes for the other.

01Composition
Written sequence Ac-Ser-Tyr-Ser-Nle-Glu-His-D-Phe-Arg-Trp-Gly-Lys-Pro-Val-NH2
Molecular formulaC78H111N21O19

This compound contains residues outside the standard twenty, so a theoretical mass cannot be derived from a one-letter sequence. The masses above are computed from the molecular formula instead.

This is not a technicality. A laboratory that computes an expected mass from a sequence lookup will get the wrong number for this compound, and will then either report a false mismatch or adjust the expectation to fit the measurement.

02 Expected electrospray ions
Positive mode, protonated
Charge m/z
[M+1H]+ 1646.844
[M+2H]2+ 823.926
[M+3H]3+ 549.619

These are the ions an electrospray source is expected to produce for a neutral mass of 1645.8365 daltons, computed from the monoisotopic mass and the proton mass. They are what a raw spectrum should show before deconvolution.

A report that gives only a deconvoluted mass without the charge states it was derived from cannot be checked. Deconvolution is a calculation, and a calculation whose inputs are not shown is an assertion.

03What goes wrong with this compound
Linear, where Melanotan II is cyclic. The two are routinely confused and differ by more than 600 daltons, so mass separates them immediately and price does not.
Norleucine and D-phenylalanine are non-proteinogenic, so a theoretical mass has to come from the formula rather than from a sequence lookup.
Tryptophan and the methionine-mimicking norleucine both make the molecule light and oxidation sensitive in solution.

Every compound fails in its own way. A generic peptide method applied without regard to these specifics produces a number that looks like a result and is not one.

Cyclic and linear analogues of alpha-MSH. Ring closure changes the mass by two daltons against the linear precursor, which is within the error of a careless measurement and not of a careful one.

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04How it is determined

LC-MS with mass accuracy sufficient to separate the cyclised product from its linear precursor, purity by RP-HPLC at 214 nm.

Where the delivered milligram figure matters rather than the percentage, net peptide content by combustion analysis and water by Karl Fischer are added, and the certificate carries a closed mass balance instead of a single purity number.

Determinations available
LC-MS identity Measured mass
RP-HPLC purity Area at 214 nm
Net peptide content Milligrams
Water content Karl Fischer
Counter-ion Ion chromatography
Bacterial endotoxins USP <85>
FAQMelanotan I

What is the molecular weight of Melanotan I?

The average molecular weight is 1646.85 daltons and the monoisotopic mass is 1645.8365 daltons, computed from the molecular formula C78H111N21O19 shown on this page. Both figures are calculated rather than transcribed, so they can be checked against the stated composition.

How is Melanotan I tested for purity and identity?

LC-MS with mass accuracy sufficient to separate the cyclised product from its linear precursor, purity by RP-HPLC at 214 nm. Purity describes the chromatographable fraction only and is not the same quantity as the peptide mass delivered per vial.

What goes wrong when Melanotan I is analysed?

Linear, where Melanotan II is cyclic. The two are routinely confused and differ by more than 600 daltons, so mass separates them immediately and price does not.

What does Melanotan I analysis cost at ANALYTON?

Peptide analysis, specialist compound is priced at 590 US dollars, covering the determinations described on this page. Content, safety and elemental determinations are ordered alongside and are priced separately on the price list.

Other compounds in this class

Updated 2026-09-01. Masses computed at build, not transcribed.