Catalogue / FOXO4-DRI
FOXO4-DRI
Also written as FOXO4 D-retro-inverso peptide.
A retro-inverso peptide built entirely from D-amino acids in reversed sequence order, at roughly 5.4 kilodaltons one of the largest synthetic peptides in circulation.
| Molecular formula | C228H388N86O64 |
| Average mass | 5358.06 Da |
| Monoisotopic mass | 5354.975 Da |
| Class | Signalling and mitochondrial peptides |
| Determination | Peptide analysis, specialist compound |
| Price | 590 USD |
Computed at build time from the composition shown on this page, not transcribed from a supplier datasheet.
FOXO4-DRI has an average molecular weight of 5358.06 daltons and a monoisotopic mass of 5354.975 daltons. Identity is confirmed by matching the measured deconvoluted mass to that figure, and purity is reported separately by reversed-phase chromatography at 214 nm. The two numbers answer different questions and neither substitutes for the other.
| Charge | m/z |
|---|---|
| [M+3H]3+ | 1785.999 |
| [M+4H]4+ | 1339.751 |
| [M+5H]5+ | 1072.002 |
These are the ions an electrospray source is expected to produce for a neutral mass of 5354.975 daltons, computed from the monoisotopic mass and the proton mass. They are what a raw spectrum should show before deconvolution.
A report that gives only a deconvoluted mass without the charge states it was derived from cannot be checked. Deconvolution is a calculation, and a calculation whose inputs are not shown is an assertion.
| Every residue is the D-isomer. Achiral chromatography cannot distinguish the intended all-D product from an all-L peptide of identical sequence, and mass cannot either: they are the same mass. Confirming configuration requires a chiral method, and a certificate that does not state one has not confirmed it. |
| At 46 residues the synthesis is long enough that deletion sequences are a genuine population rather than a rounding error. Purity in the low nineties is normal for honest material. |
| Highly cationic, so the electrospray envelope spreads across many charge states and deconvolution parameters must be reported for the neutral mass to be reproducible. |
Every compound fails in its own way. A generic peptide method applied without regard to these specifics produces a number that looks like a result and is not one.
Endogenous peptides with defined sequences, mostly unmodified, where the analytical question is simply whether the delivered material is the stated sequence at the stated mass.
LC-MS identity with deconvolution and RP-HPLC purity at 214 nm.
Where the delivered milligram figure matters rather than the percentage, net peptide content by combustion analysis and water by Karl Fischer are added, and the certificate carries a closed mass balance instead of a single purity number.
| LC-MS identity | Measured mass |
| RP-HPLC purity | Area at 214 nm |
| Net peptide content | Milligrams |
| Water content | Karl Fischer |
| Counter-ion | Ion chromatography |
| Bacterial endotoxins | USP <85> |
What is the molecular weight of FOXO4-DRI?
The average molecular weight is 5358.06 daltons and the monoisotopic mass is 5354.975 daltons, computed from the molecular formula C228H388N86O64 shown on this page. Both figures are calculated rather than transcribed, so they can be checked against the stated composition.
How is FOXO4-DRI tested for purity and identity?
LC-MS identity with deconvolution and RP-HPLC purity at 214 nm. Purity describes the chromatographable fraction only and is not the same quantity as the peptide mass delivered per vial.
What goes wrong when FOXO4-DRI is analysed?
Every residue is the D-isomer. Achiral chromatography cannot distinguish the intended all-D product from an all-L peptide of identical sequence, and mass cannot either: they are the same mass. Confirming configuration requires a chiral method, and a certificate that does not state one has not confirmed it.
What does FOXO4-DRI analysis cost at ANALYTON?
Peptide analysis, specialist compound is priced at 590 US dollars, covering the determinations described on this page. Content, safety and elemental determinations are ordered alongside and are priced separately on the price list.
Other compounds in this class
Updated 2026-09-01. Masses computed at build, not transcribed.