Catalogue / Dihexa
Dihexa
Also written as N-hexanoic-Tyr-Ile-(6)-aminohexanoic amide.
A lipidated dipeptide derivative rather than a peptide in the ordinary sense, carrying a hexanoyl group at one end and an aminohexanoic amide at the other.
| Molecular formula | C27H44N4O5 |
| Average mass | 504.66 Da |
| Monoisotopic mass | 504.3312 Da |
| Written as | Hexanoyl-Tyr-Ile-aminohexanoic acid amide |
| Class | Signalling and mitochondrial peptides |
| Determination | Peptide analysis, specialist compound |
| Price | 590 USD |
Computed at build time from the composition shown on this page, not transcribed from a supplier datasheet.
Dihexa has an average molecular weight of 504.66 daltons and a monoisotopic mass of 504.3312 daltons. Identity is confirmed by matching the measured deconvoluted mass to that figure, and purity is reported separately by reversed-phase chromatography at 214 nm. The two numbers answer different questions and neither substitutes for the other.
| Written sequence | Hexanoyl-Tyr-Ile-aminohexanoic acid amide |
| Molecular formula | C27H44N4O5 |
This compound contains residues outside the standard twenty, so a theoretical mass cannot be derived from a one-letter sequence. The masses above are computed from the molecular formula instead.
This is not a technicality. A laboratory that computes an expected mass from a sequence lookup will get the wrong number for this compound, and will then either report a false mismatch or adjust the expectation to fit the measurement.
| Charge | m/z |
|---|---|
| [M+1H]+ | 505.338 |
| [M+2H]2+ | 253.173 |
These are the ions an electrospray source is expected to produce for a neutral mass of 504.3312 daltons, computed from the monoisotopic mass and the proton mass. They are what a raw spectrum should show before deconvolution.
A report that gives only a deconvoluted mass without the charge states it was derived from cannot be checked. Deconvolution is a calculation, and a calculation whose inputs are not shown is an assertion.
| Only two of the building blocks are amino acids. Sequence-based reasoning does not apply and the theoretical mass comes from the formula. |
| The hexanoyl amide is hydrolysable. Loss of it gives a species 98 daltons lighter that has none of the lipophilicity the compound is made for. |
| Strongly lipophilic for its size, so it adsorbs to plastic and needs a solvent system that keeps it in solution through the whole preparation. |
Every compound fails in its own way. A generic peptide method applied without regard to these specifics produces a number that looks like a result and is not one.
Endogenous peptides with defined sequences, mostly unmodified, where the analytical question is simply whether the delivered material is the stated sequence at the stated mass.
LC-MS identity with deconvolution and RP-HPLC purity at 214 nm.
Where the delivered milligram figure matters rather than the percentage, net peptide content by combustion analysis and water by Karl Fischer are added, and the certificate carries a closed mass balance instead of a single purity number.
| LC-MS identity | Measured mass |
| RP-HPLC purity | Area at 214 nm |
| Net peptide content | Milligrams |
| Water content | Karl Fischer |
| Counter-ion | Ion chromatography |
| Bacterial endotoxins | USP <85> |
What is the molecular weight of Dihexa?
The average molecular weight is 504.66 daltons and the monoisotopic mass is 504.3312 daltons, computed from the molecular formula C27H44N4O5 shown on this page. Both figures are calculated rather than transcribed, so they can be checked against the stated composition.
How is Dihexa tested for purity and identity?
LC-MS identity with deconvolution and RP-HPLC purity at 214 nm. Purity describes the chromatographable fraction only and is not the same quantity as the peptide mass delivered per vial.
What goes wrong when Dihexa is analysed?
Only two of the building blocks are amino acids. Sequence-based reasoning does not apply and the theoretical mass comes from the formula.
What does Dihexa analysis cost at ANALYTON?
Peptide analysis, specialist compound is priced at 590 US dollars, covering the determinations described on this page. Content, safety and elemental determinations are ordered alongside and are priced separately on the price list.
Other compounds in this class
Updated 2026-09-01. Masses computed at build, not transcribed.