Catalogue / Argireline
Argireline
Also written as Acetyl hexapeptide-8, Acetyl hexapeptide-3.
An N-acetylated, C-amidated hexapeptide modelled on the SNAP-25 N-terminal sequence, and the most widely used cosmetic peptide active.
| Molecular formula | C34H60N14O12S |
| Average mass | 888.99 Da |
| Monoisotopic mass | 888.4236 Da |
| Residues | 6 |
| Termini | N-terminal acetyl, C-terminal amide |
| Class | Cosmetic active peptides |
| Determination | Peptide analysis, catalogue compound |
| Price | 341 USD |
Computed at build time from the composition shown on this page, not transcribed from a supplier datasheet.
Argireline has an average molecular weight of 888.99 daltons and a monoisotopic mass of 888.4236 daltons. Identity is confirmed by matching the measured deconvoluted mass to that figure, and purity is reported separately by reversed-phase chromatography at 214 nm. The two numbers answer different questions and neither substitutes for the other.
| One letter | EEMQRR |
| Three letter | Glu-Glu-Met-Gln-Arg-Arg |
| Modifications | N-terminal acetyl, C-terminal amide |
The formula and both masses in the table above are derived from this sequence and these terminal modifications. A reader who believes a figure is wrong can check the composition rather than take the number on trust, which is the point of publishing it this way.
A sequence is not an identity result. It is the expected composition against which a measured mass is compared, and the comparison is what appears on the certificate.
| Charge | m/z |
|---|---|
| [M+1H]+ | 889.431 |
| [M+2H]2+ | 445.219 |
These are the ions an electrospray source is expected to produce for a neutral mass of 888.4236 daltons, computed from the monoisotopic mass and the proton mass. They are what a raw spectrum should show before deconvolution.
A report that gives only a deconvoluted mass without the charge states it was derived from cannot be checked. Deconvolution is a calculation, and a calculation whose inputs are not shown is an assertion.
| Supplied commercially as a dilute aqueous solution, typically at 0.05 percent active. A certificate that reports the solution concentration as the peptide purity is comparing two different things. |
| In a finished emulsion the peptide partitions into the aqueous phase and the extraction step decides the result. Spike recovery in the actual formulation is mandatory. |
| Methionine oxidation adds 16 daltons and preservatives in the base co-elute in the same region. |
Every compound fails in its own way. A generic peptide method applied without regard to these specifics produces a number that looks like a result and is not one.
Supplied as raw actives or already formulated into an emulsion. In a finished cosmetic the matrix, not the peptide, decides whether the determination works.
LC-MS or HPLC-UV against a certified reference standard with matrix-matched calibration and spike recovery, because emulsifiers and pigments co-extract and suppress ionisation.
Where the delivered milligram figure matters rather than the percentage, net peptide content by combustion analysis and water by Karl Fischer are added, and the certificate carries a closed mass balance instead of a single purity number.
| LC-MS identity | Measured mass |
| RP-HPLC purity | Area at 214 nm |
| Net peptide content | Milligrams |
| Water content | Karl Fischer |
| Counter-ion | Ion chromatography |
| Bacterial endotoxins | USP <85> |
What is the molecular weight of Argireline?
The average molecular weight is 888.99 daltons and the monoisotopic mass is 888.4236 daltons, computed from the sequence EEMQRR shown on this page. Both figures are calculated rather than transcribed, so they can be checked against the stated composition.
How is Argireline tested for purity and identity?
LC-MS or HPLC-UV against a certified reference standard with matrix-matched calibration and spike recovery, because emulsifiers and pigments co-extract and suppress ionisation. Purity describes the chromatographable fraction only and is not the same quantity as the peptide mass delivered per vial.
What goes wrong when Argireline is analysed?
Supplied commercially as a dilute aqueous solution, typically at 0.05 percent active. A certificate that reports the solution concentration as the peptide purity is comparing two different things.
What does Argireline analysis cost at ANALYTON?
Peptide analysis, catalogue compound is priced at 341 US dollars, covering the determinations described on this page. Content, safety and elemental determinations are ordered alongside and are priced separately on the price list.
Updated 2026-09-01. Masses computed at build, not transcribed.